Comparison of Aflibercept Producers Derived from СНО and СНО-GS Cell Lines

When creating cell lines expressing recombinant proteins, the type of selection marker and selection conditions are of essential importance. In this work, we aim to obtain and compare cell lines based on CHO and CHO-GS as potential aflibercept producers to identify the most suitable cell line for obtain ing an aflibercept biosimilar. As a result, 10 monoclonal cell lines-producers of a potential aflibercept biosimilar were obtained based on СНО and CHO-GS cell platforms. In the two groups, the productivity on the 15th day of periodic cultivation with feeding achieved 2.5 g/L. The СНО-GS group showed an in crease in the specific cell productivity of aflibercept producers. The conclusion is made that the СНО-GS glutamine auxotroph line is a more preferable option. This cell line enables a comparable yield of target protein at lower concentrations of viable cells, which has a favorable effect on further stages of purification and isolation of target protein.