Support-Free Implantable Photoelectrochemical Hydrogel Fiber Enables Long-Term Monitoring in Free-Behaving Organisms

The development of long-term and in situ in vivo monitoring techniques is critical for environmental biology, life sciences, and analytical chemistry. However, existing in vivo analysis methods are limited by the complex and large instruments or adverse impacts of rigid implanted substrates on living organisms, making it difficult to achieve continuous in situ detection. Herein, taking advantage of the flexibility and biocompatibility of the hydrogel fiber and solving its instability or opacity problems caused by ionic or polymer conduction for hydrogel fibers, a photoelectrochemical (PEC) hydrogel fiber free of conventional rigid substrate support is successfully prepared and achieves long-term tracking of persistent organic pollutants in free-behaving fish, timely identifying their environmental ecological risks. This support-free PEC fiber exhibits fascinating properties of electrical and light conductivity, flexibility, antifouling ability, and biocompatibility, allowing it to be implanted in vivo for 70 days without experiencing significant loss of sensing performance and causing apparent inflammation and immune responses. Moreover, the fabricated fiber not only achieves in vitro pentachlorophenol detection with high selectivity, low detection limit, good reproducibility, and dual-mode sensing but also realizes in vivo monitoring of pentachlorophenol enriched in fish brain for up to 70 days with satisfactory reliability, unraveling its tempting potential for various in vivo application.