Photon-counting Raman spectroscopy at a MHz spectral rate for biochemical imaging of an entire organism

Raman spectroscopy, which probes fine molecular vibrations, is crucial for interpreting covalent bonds, chemical compositions, and other molecular dynamics in mixtures via their vibrational fingerprint signatures. However, over the past few decades, longstanding barriers have been encountered in both the sensitivity and speed of Raman spectroscopy, limiting its ability to be extended to broader biochemical applications. Here, we introduce a versatile analytical workhorse, the fiber-array Raman engine (termed FIRE). In FIRE, a distinctive fiber array bundle delays the Raman shifts at a scale of 3-960 ns, and a highly dynamic single-channel photon-counting detector achieves spectral measurements that outperform the best commercial confocal Raman microscope. Crucially, FIRE features a major advantage of nonrepetitive single-shot spectra measurement at a MHz repetition rate with a full Raman span (-300-4300 cm<sup>-1</sup>) covering the fingerprint, silent, C-H, and O-H regions and therefore represents a major step toward overall improving of sensitivity, speed, and spectral span. We demonstrate full Raman spectral imaging of the metabolic activity of intact Caenorhabditis elegans. FIRE exhibits superior performance to a Raman microscope in all aspects, including autofluorescence suppression, and will elucidate a variety of biochemical applications.

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Publication Details

Journal
Nature Communications
Published
2025-04-23
DOI
https://doi.org/10.1038/s41467-025-59030-8
Citations
3
Primary Topic
Spectroscopy Techniques in Biomedical and Chemical Research
Type
article
Field-Weighted Citation Impact
4.90

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article

Photon-counting Raman spectroscopy at a MHz spectral rate for biochemical imaging of an entire organism

Long Xiao, Wanjun Lu, Ping Wang, Shuai Yan et al.
3 citations
Nature Communications
Spectroscopy Techniques in Biomedical and Chemical Research
4.90
article

Photon-counting Raman spectroscopy at a MHz spectral rate for biochemical imaging of an entire organism

Long Xiao, Wanjun Lu, Ping Wang, Shuai Yan, Qi Zhang, Xingbo Liu, Bi Zhang, Sicheng Li, Yage Chen, Shuai Wang, Aiguo Shen, Zhiliang Huang, Haozheng Li, Jianfeng Liu, Zhe Wang, Yiran Li, Qipei Zhou, Xin Lv
article en
3 citations

Abstract

Raman spectroscopy, which probes fine molecular vibrations, is crucial for interpreting covalent bonds, chemical compositions, and other molecular dynamics in mixtures via their vibrational fingerprint signatures. However, over the past few decades, longstanding barriers have been encountered in both the sensitivity and speed of Raman spectroscopy, limiting its ability to be extended to broader biochemical applications. Here, we introduce a versatile analytical workhorse, the fiber-array Raman engine (termed FIRE). In FIRE, a distinctive fiber array bundle delays the Raman shifts at a scale of 3-960 ns, and a highly dynamic single-channel photon-counting detector achieves spectral measurements that outperform the best commercial confocal Raman microscope. Crucially, FIRE features a major advantage of nonrepetitive single-shot spectra measurement at a MHz repetition rate with a full Raman span (-300-4300 cm<sup>-1</sup>) covering the fingerprint, silent, C-H, and O-H regions and therefore represents a major step toward overall improving of sensitivity, speed, and spectral span. We demonstrate full Raman spectral imaging of the metabolic activity of intact Caenorhabditis elegans. FIRE exhibits superior performance to a Raman microscope in all aspects, including autofluorescence suppression, and will elucidate a variety of biochemical applications.

Nature CommunicationsVol. 16(1)
Cheung Kong Graduate School of Business (CN), China University of Geosciences (CN), Wuhan Textile University (CN), Wuhan National Laboratory for Optoelectronics (CN), Huaiyin Institute of Technology (CN), Huazhong University of Science and Technology (CN), Hubei University of Technology (CN)
National Natural Science Foundation of China, Wuhan National Laboratory for Optoelectronics, National Natural Science Foundation of China-Yunnan Joint Fund
Openalex Percentile: Top 6%
Spectroscopy Techniques in Biomedical and Chemical Research
4.90
DOIOpen Access PDFOpenAlex
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