Rapid detection of <scp>ACCase W2027C</scp> mutation in <scp><i>Leptochloa chinensis</i></scp> (<scp>L</scp>.) Nees using <scp>RAA</scp>‐<scp>CRISPR</scp>/Cas12a: a sensitive and visual tool for herbicide resistance diagnosis
This report has described the application of the RAA-CRISPR system for mutation detection in herbicide resistant weeds, presenting a promising tool for integrated weed management and enabling more timely decision-making regarding herbicide application and resistance management. © 2025 Society of Chemical Industry.
Authors
- Chao Zhang (ORCID: https://orcid.org/0000-0002-1714-8381)
- Ziheng Cao (ORCID: https://orcid.org/0009-0008-5748-505X)
- Lang Pan (ORCID: https://orcid.org/0000-0001-8248-2055)
- Shu Liu (ORCID: https://orcid.org/0000-0002-8848-6871)
- Jiahao Lun
Institutions
- Anhui Agricultural University (CN)
- Yueyang Changling Equipment Research Institute (China) (CN)
- Hunan Agricultural University (CN)
Publication Details
- Journal
- Pest Management Science
- Published
- 2025-04-23
- DOI
- https://doi.org/10.1002/ps.8840
- Citations
- 2
- Primary Topic
- CRISPR and Genetic Engineering
- Type
- article
- Field-Weighted Citation Impact
- 1.61
Funders
- Natural Science Foundation of Hunan Province
- Earmarked Fund for China Agriculture Research System
- National Key Research and Development Program of China