Differences in Carbon and Nitrogen Cycling Strategies and Regional Variability in Biological Soil Crust Types

Biological soil crusts (BSCs) play a pivotal role in maintaining ecosystem stability and soil fertility in arid and semi-arid regions. However, the biogeographical differences in soil functional composition between cyanobacterial BSCs (C-BSCs) and moss BSCs (M-BSCs), particularly how environmental changes affect nutrient cycling strategies and microbial community functions, remain poorly understood. In this study, we investigated BSCs across aridity gradients (semi-humid, semi-arid, and arid regions) in China, focusing on carbon and nitrogen cycling pathways, enzyme activities, and nutrient acquisition strategies. It was found that aridity and BSC type had significant effects on the functional characteristics of microorganisms. This was demonstrated by significant differences in various soil microbial activities including enzyme activities and carbon and nitrogen nutrient cycling. With increasing aridity, C-BSCs exhibited reduced carbon cycling activity but enhanced nitrogen cycling processes, whereas M-BSCs displayed diminished activity in both carbon and nitrogen cycling. These divergent strategies were linked to soil properties such as pH and organic carbon content, with C-BSCs adapting through nitrogen-related processes (e.g., <i>nifH</i>, <i>amoA</i>) and M-BSCs relying on C fixation and degradation. These findings provide novel insights into the functional gene diversity of BSCs across different regions, offering valuable references for ecological restoration in arid areas. Specifically, our study highlights the potential of BSC inoculation for carbon and nitrogen enrichment in arid regions, with implications for climate-resilient restoration practices.