CRISPR mutant rapid identification in B. napus: RNA-Seq functional profiling and breeding technology application

In this study, The <i>Bnaida</i> mutant by gene editing in the cv ZS11, which is widely used in rapeseed breeding. The phenotypic analysis showed that the petal was attached to the pod and pods were harder to crack in edited plants, and then we quickly introduced two <i>Bnaida</i> loci into the elite line of SW1-6 by backcrossing with edited ZS11 as the donor plant. Locus-specific primer combinations were designed to differentiate heterozygous and homozygous genotypes in backcrossing generations, enabling efficient and rapid selection. This study highlights the integration of gene editing and genotyping selection, offering insights into the future of gene editing-assisted breeding.