Optimisation of cutaneous microbiota sampling methodology
Sampling approaches are critical for the success of sequencing. The conditions investigated in this study did not influence microbiome profiling allowing consistent sampling of the microbiota. However, data clustering was affected more by individual subject than by the conditions investigated, suggesting the importance of recognizing inter-individual variability as an important factor in real-life skin microbiome studies.
Authors
- Adrian Heagerty (ORCID: https://orcid.org/0000-0003-4351-7467)
- Iain Chapple (ORCID: https://orcid.org/0000-0003-2697-7082)
- Dario Leonardo Balacco (ORCID: https://orcid.org/0000-0002-7648-1213)
- Sarah A. Kuehne (ORCID: https://orcid.org/0000-0001-6790-8433)
- Melissa M. Grant (ORCID: https://orcid.org/0000-0003-1154-7266)
- Ajoy Bardhan (ORCID: https://orcid.org/0000-0001-8281-6123)
- Josefine Hirschfeld (ORCID: https://orcid.org/0000-0001-8512-7411)
- Hebatallah Ibrahim
Institutions
- Suez Canal University (EG)
- University Hospitals Birmingham NHS Foundation Trust (GB)
- University of Bonn (DE)
- Birmingham Community Healthcare NHS Trust (GB)
- NIHR Birmingham Biomedical Research Centre (GB)
- Nottingham Trent University (GB)
- University of Birmingham (GB)
Publication Details
- Journal
- Frontiers in Microbiomes
- Published
- 2025-04-22
- DOI
- https://doi.org/10.3389/frmbi.2025.1559981
- Citations
- 1
- Primary Topic
- Dermatology and Skin Diseases
- Type
- article
- Field-Weighted Citation Impact
- 2.00
Funders
- Dystrophic Epidermolysis Bullosa Research Association