Establishment of <i>in vitro</i> culture conditions and acclimatization for mass production in <i>Persicaria chinensis</i> (L.) H. Gross

For mass production of Persicaria chinensis, growth regulators and acclimatization soil for regenerated plants were selected.For shoot proliferation, Murashige and Skoog (MS) medium with 2 mg/L of 6-benzylaminopurine was found to be the most effective, achieving a shoot induction rate of 95.8%, with an average of 3.5 shoots developed and a shoot length of 10.50 mm.The most effective medium and growth regulator for rooting was MS media with 2 mg/L of indole-3-butyric acid, resulting in a rooting rate of 100%, an average of 4.9 roots, and a root length of 75.75 mm.Horticultural substrate was found to be the most effective for ex vitro acclimatization of regenerated plants, with a seedling length of shoots of 58.42 mm and a length of roots of 190.10 mm.These results confirmed that P. chinensis can be mass propagated using in vitro culture.