Mining Arylsulfatase from Genome-Scale Metabolic Pathways of Pseudoalteromonas sp. SR43-6 and Its Agar-Based Desulfurization Applications
Arylsulfatase catalyzes the cleavage of sulfate ester bonds and plays a role in agar desulfation, thereby enhancing agar gel strength and quality. While studying the desulfurization pathway in <i>Pseudoalteromonas</i>sp. SR43-6, a sequence encoding a potential arylsulfatase-<i>Pseudoalteromonas</i> Ars (<i>Ps</i>-Ars)-was found. The enzyme, with <i>p</i>-nitrophenyl sulfate as a substrate, exhibited optimal activity at 35 °C and pH 8.0. Its relative activity (206 U/mg) exceeded that of the recently identified arylsulfatases. Four hundred units of the enzyme removed 86.4% of sulfate groups from <i>Gelidium amansii</i> agar in 4 h, whereas 800 U of the enzyme removed 71.3% of sulfate groups from <i>Gracilaria lemaneiformis</i> agar in 8 h. After enzymatic treatment, <i>G. amansii</i> agar gel strength was enhanced by 32%, and a similar improvement was observed in <i>G. lemaneiformis</i> agar gel strength. Enzymatic agar desulfurization offers mild, quality-retaining, and environmentally friendly advantages, augmenting industrial application prospects.
Authors
- Songzhi Li
- Minxiao Wang (ORCID: https://orcid.org/0000-0002-6567-3295)
- Xiangzhao Mao (ORCID: https://orcid.org/0000-0002-6315-1338)
- Hong Jiang (ORCID: https://orcid.org/0000-0003-2939-2514)
- Jiayu Li (ORCID: https://orcid.org/0009-0007-2760-3433)
- Huan Zhang (ORCID: https://orcid.org/0000-0001-6615-1627)
- Jie Li (ORCID: https://orcid.org/0000-0002-5131-4769)
- Yanli Chen (ORCID: https://orcid.org/0000-0002-0350-7160)
- Yang Guo (ORCID: https://orcid.org/0009-0000-7581-7865)
Institutions
- Institute of Environmental Engineering (PL)
- Institute of Oceanology (BG)
- Qingdao University of Technology (CN)
- Ocean University of China (CN)
Publication Details
- Journal
- ACS Omega
- Published
- 2025-04-22
- DOI
- https://doi.org/10.1021/acsomega.5c01356
- Citations
- 1
- Primary Topic
- Enzyme Production and Characterization
- Type
- article
- Field-Weighted Citation Impact
- 1.23
Funders
- National Natural Science Foundation of China
- Natural Science Foundation of Hainan Province
- Natural Science Foundation of Shandong Province
- Taishan Scholar Foundation of Shandong Province
- Major Scientific and Technological Innovation Project of Shandong Province
- National Key Research and Development Program of China
- Fundamental Research Funds for the Central Universities
- Science and Technology Support Plan for Youth Innovation of Colleges and Universities of Shandong Province of China