Effect of new bioactive substances obtained from possible probiotic bacteria on miRNA expression by in vitro cocultivation in colorectal cancer cells

In our study, were evaluated effect on colorectal cancer cells of possible probiotic bacteria and new bioactive substances (paraprobiotics/postbiotics), miRNA-expression. <i>Lactobacillus rhamnosus, Lactobacillus reuteri</i> strains, HT-29, and Caco-2 were used. The cytotoxicity of the biotics was determined by MTT and miRNA expression. In line with the data obtained, it was determined that probiotics had a proliferative effect on the fibroblast cell line and a cytotoxic effect on cancer cells. It was observed that paraprobiotics had a minimal effect compared to probiotics, postbiotics had a greater effect. In this work, the activities of new pharmabiotics are compared with living cells, eliminating the limitations of probiotics in terms of shelf life and viability, and thus allowing the creation of new commercial domestic and national products, which increases the unique value of our study. In addition, there will be safer alternatives for suppressed immune systems, and various disadvantages of probiotics can be eliminated.