Exploring biosurfactant production in Dyadobacter fermentans JDP9: Insights from genome sequencing and analysis

The present study examines the comprehensive analysis of the whole genome shotgun sequencing of Dyadobacter fermentans JDP9. The Dyadobacter genus is recognized for its synthesis of flexirubin; however, its capacity for biosurfactant (BS) production, particularly glycoprotein BS, has not been reported yet. A potential BS-producing D. fermentans JDP9 strain was isolated from hydrocarbon-contaminated soil. The BS was primarily characterized as a glycoprotein. The utilization of the Illumina platform for whole genome shotgun sequencing resulted in 2,469,724 high-quality reads assembled into 27 scaffolds, revealing a genome size of 7,979,294, GC content of 50.3 %, a Scaffold L50 value of 5, and a Scaffold N50 value of 522,766. The NCBI Prokaryotic Genome Annotation Pipeline identified 6,602 genes and 6,557 CDS. The systematic examination carried out by AntiSMASH, identified a total of 9 clusters, specifically the non-ribosomal peptide synthetase gene cluster. Herein, we proposed that the glycoprotein BS production is facilitated by enzymes involved in the dTDP-L-rhamnose biosynthetic pathway, including glucose-1-phosphate thymidylyltransferase, dTDP-glucose 4,6-dehydratase, and dTDP-4-dehydrorhamnose reductase present in the JDP9 strain genome. These enzymes contribute to generating dTDP-L-rhamnose, a crucial sugar moiety for glycosylation reactions. This study establishes a foundation for prospective investigations into glycoprotein BS production, presenting potential biotechnology implications. • Dyadobacter fermentans JDP9 has been characterized as a novel glycoprotein biosurfactant-producer. • Whole Genome Shotgun sequencing elucidated a genome size of 7.98 Mb, encompassing 6,557 annotated coding sequences. • Critical enzymes of the dTDP-L-rhamnose biosynthetic pathway were identified, facilitating glycosylation for glycoprotein-BS synthesis. • NRPS-like gene cluster and siderophore regulators likely control biosurfactant synthesis and its glycosylation process. • The investigation offers foundational insights into the biosynthesis of glycoprotein biosurfactant.