A strategy of extracting and purifying the α-terpineol obtained from the <i>Penicillium digitatum</i> biotransformation of limonene

α-Terpineol, an abundant oxygenated monoterpene compound, shows a varied range of beneficial bioactivities. This study focused on the efficient extraction and purification of α-terpineol from the biotransformation of limonene using <i>Penicillium digitatum</i> DSM 62840 mutant (<i>PdTP1</i>-overexpressed OE2 strain). The α-terpineol was primarily distributed in the supernatant, with minimal association with the biomass. And the optimal extraction was achieved using ethyl acetate as the extractant, which can directly obtain the highest recovery of α-terpineol without sequential extractions. The equilibrium of mass transfer was quickly reached (≤20 s in vortex). Additionally, the purification method of α-terpineol using column chromatography was described, further improving the purity of product to 96.86% in gas chromatography. Structural identification of purified α-terpineol was confirmed using gas chromatography-mass spectrometry, Fourier-transform infrared spectroscopy, and nuclear magnetic resonance. This simplified and efficient strategy for the extraction and purification of α-terpineol not only provides a solid theoretical basis for the final step in the relevant study of microbial synthesis of α-terpineol but also is of great significance for its industrial application.

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Publication Details

Journal
Preparative Biochemistry & Biotechnology
Published
2025-04-22
DOI
https://doi.org/10.1080/10826068.2025.2494102
Citations
2
Primary Topic
Enzyme Catalysis and Immobilization
Type
article
Field-Weighted Citation Impact
1.61

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article

A strategy of extracting and purifying the α-terpineol obtained from the Penicillium digitatum biotransformation of limonene

Feng Yingjie, Aiqun Yu, Yufeng Fu, Ding Meizhou et al.
2 citations
Preparative Biochemistry & Biotechnology
Enzyme Catalysis and Immobilization
1.61
article

A strategy of extracting and purifying the α-terpineol obtained from the Penicillium digitatum biotransformation of limonene

Feng Yingjie, Aiqun Yu, Yufeng Fu, Ding Meizhou, Qiuling Wang, Gang Fan, Haoliang Li, Yujiao Cheng, Zixuan Wang, Jian Feng, Linhua Huang, Yongfeng Yang, Lu‐Lu Zhang, Guijie Li, Jinchu Yang, Weimin Song, Zhenzhen Huang, Yongming Xu, Jingnan Ren, Zehao Li
article en
2 citations

Abstract

α-Terpineol, an abundant oxygenated monoterpene compound, shows a varied range of beneficial bioactivities. This study focused on the efficient extraction and purification of α-terpineol from the biotransformation of limonene using <i>Penicillium digitatum</i> DSM 62840 mutant (<i>PdTP1</i>-overexpressed OE2 strain). The α-terpineol was primarily distributed in the supernatant, with minimal association with the biomass. And the optimal extraction was achieved using ethyl acetate as the extractant, which can directly obtain the highest recovery of α-terpineol without sequential extractions. The equilibrium of mass transfer was quickly reached (≤20 s in vortex). Additionally, the purification method of α-terpineol using column chromatography was described, further improving the purity of product to 96.86% in gas chromatography. Structural identification of purified α-terpineol was confirmed using gas chromatography-mass spectrometry, Fourier-transform infrared spectroscopy, and nuclear magnetic resonance. This simplified and efficient strategy for the extraction and purification of α-terpineol not only provides a solid theoretical basis for the final step in the relevant study of microbial synthesis of α-terpineol but also is of great significance for its industrial application.

Preparative Biochemistry & BiotechnologyVol. 55(9)
Tianjin University of Science and Technology (CN), Southwest University (CN), Huazhong Agricultural University (CN), China Tobacco (CN), Henan University of Technology (CN), Citrus Research Institute (CN), Essence Line (Czechia) (CZ)
National Natural Science Foundation of China, China Postdoctoral Science Foundation
Openalex Percentile: Top 18%
Enzyme Catalysis and Immobilization
1.61
DOISourceOpenAlex
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