Stachybotrins G and H, Two New Phenylspirodrimane Derivatives from the Fungus Stachybotrys chartarum

Two new phenylspirodrimane derivatives, designated as stachybotrins G and H (1: and 2: ), which feature an <i>N</i>-isobutyl side chain, along with four known analogues (3: -6: ), were isolated from the fungus <i>Stachybotrys chartarum</i>. All the structures were determined through comprehensive spectroscopic analyses, primarily based on HRESIMS and NMR data. The antibacterial activity of all isolated compounds was evaluated. Compound 5: demonstrated antibacterial activity against the Gram-positive bacterium <i>Staphylococcus aureus</i> ATCC 6538, with a minimum inhibitory concentration (MIC) value of 6.25 µg/mL.