Aptamer-Based Microfluidic Assay for In-Field Detection of Salicylic Acid in Botrytis cinerea-Infected Strawberries
Rapid detection of plant infections is crucial for minimising crop loss and optimising management strategies, particularly in the context of climate change. While traditional diagnostic methods provide precise measurements of phytohormones such as salicylic acid (SA), a key regulator of plant defence responses, their reliance on bulky equipment and lengthy analysis times limits field applicability. This study presents a microfluidic-based aptamer assay for SA detection, enabling rapid and sensitive fluorescence-based readout from plant samples. A tailored sample pre-treatment protocol was developed and validated with real strawberry samples using HPLC measurements. The assay demonstrated a detection limit ranging from 10<sup>-9</sup> to 10<sup>-6</sup> mg/mL, within the relevant range for early infection diagnosis. The integration of the microfluidic platform with the optimised pre-treatment protocol offers a portable, cost-effective solution for on-site phytohormone analysis, providing a valuable tool for early infection detection and improved crop management.
Authors
- Ana Margarida Fortes (ORCID: https://orcid.org/0000-0001-7552-0164)
- Rafaela R. da Rosa (ORCID: https://orcid.org/0000-0003-1767-3511)
- V. Chu (ORCID: https://orcid.org/0000-0002-5306-4409)
- Cristiana Domingues (ORCID: https://orcid.org/0000-0003-1000-1181)
- J. P. Conde (ORCID: https://orcid.org/0000-0002-5677-3024)
- Rodolfo G. Rodrigues (ORCID: https://orcid.org/0009-0007-7656-7950)
Institutions
- University of Lisbon (PT)
- Instituto de Engenharia de Sistemas e Computadores Microsistemas e Nanotecnologias (PT)
- Instituto Superior Técnico (PT)
Publication Details
- Journal
- Biosensors
- Published
- 2025-04-22
- DOI
- https://doi.org/10.3390/bios15050266
- Citations
- 1
- Primary Topic
- Plant Virus Research Studies
- Type
- article
- Field-Weighted Citation Impact
- 1.75
Funders
- Fundação para a Ciência e a Tecnologia