Comparative evaluation of proteinase K and dithiothreitol as pretreatments for extracting nucleic acids from respiratory samples for multiplex PCR
PK and DTT exhibited similar pretreatment effects on BALF samples, with neither having an impact on the results. However, DTT was superior to PK in reducing interference and enhancing the sensitivity of M-PCR for bacterial detection in sputum samples, making it the preferred pretreatment for sputum.
Authors
- Yi‐Tang Chang (ORCID: https://orcid.org/0000-0003-2826-7340)
- Yue-Ru Xie (ORCID: https://orcid.org/0000-0002-6073-4490)
- Yanfang Niu
- Ting Li (ORCID: https://orcid.org/0009-0007-8797-2353)
- Xiaodong Guan
Institutions
- Yuncheng University (CN)
Publication Details
- Journal
- BMC Microbiology
- Published
- 2025-04-23
- DOI
- https://doi.org/10.1186/s12866-025-03956-y
- Citations
- 2
- Primary Topic
- Molecular Biology Techniques and Applications
- Type
- article
- Field-Weighted Citation Impact
- 1.61
Funders
- Shanxi Medical University