Ammonicoccus fulvus gen. nov., sp. nov., a novel member of the family Propionibacteriaceae, isolated from pig manure

A Gram-positive, aerobic, non-motile coccoid bacterium, designated strain ZF39^T, was isolated from a farm in Quandian Village, Zhengzhou City, Henan Province, China, and subjected to taxonomic characterization. Optimal growth occurred at 32 ℃, pH 7.0 and 0-1% NaCl (w/v). Mannose and glucose were identified as the sugar components in the whole-cell hydrolysates. The strain's peptidoglycan type was classified as A3<i>γ</i> (ll-Dpm-Gly), and MK-9(H₄) was identified as the predominant respiratory quinone. Aminoglycolipid, aminophospholipid, glycolipids, phosphatidylglycerol and phospholipid were detected as the major polar lipids. The predominant cellular fatty acids were anteiso-C_{15 : 0} (48.7%) and iso-C_{15 : 0} (20.2%). Phylogenetic analysis based on 16S rRNA gene sequences placed strain ZF39^T within the family <i>Propionibacteriaceae</i>. The strain formed a distinct genetic lineage adjacent to the genus <i>Desertihabitans</i>, with 16S rRNA gene sequence similarities of 94.70% to <i>Desertihabitans aurantiacus</i> CPCC 204711^T and 94.56% to <i>Desertihabitans brevis</i> KCTC 49116^T. The genomic G+C content was 67.7 mol%. Genome comparisons revealed that the average nucleotide identity values between strain ZF39^T and <i>D. aurantiacus</i> CPCC 204711^T, <i>D. brevis</i> KCTC 49116^T, <i>Granulicoccus phenolivorans</i> DSM 17626^T were 72.50%, 71.98% and 72.36%, respectively. Moreover, phylogenetic analysis and phenotypic characterization supported the classification of strain ZF39^T as a novel species of a new genus within the family <i>Propionibacteriaceae</i>. The name <i>Ammonicoccus fulvus</i> gen. nov., sp. nov. is proposed, with ZF39^T (=CGMCC 1.60047^T=KCTC 59250^T=MCCC 1K08851^T) as the type strain.