A novel CRISPR-Cas9 nickase-mediated rolling circle amplification (CRIRCA) technique for gene identification and quantitative analysis of extrachromosomal DNA
Therefore, CRIRCA can simultaneously obtain the information from structure, sequence and quantitation of ecDNA. This work will fill the gap in the current research on the early monitoring of cancer targeting ecDNA, and provide support for the accurate diagnosis and treatment of cancer.
Authors
- Yuchen Song (ORCID: https://orcid.org/0000-0002-6609-4877)
- Chaoyang Guan
- Liqiang Luo (ORCID: https://orcid.org/0000-0002-5551-3861)
- Chang Feng (ORCID: https://orcid.org/0000-0002-8738-8831)
- Guifang Chen
- Peng‐Fei Li (ORCID: https://orcid.org/0000-0001-5228-6192)
- Yiming Xu (ORCID: https://orcid.org/0000-0002-4067-1373)
- Yue Zhang (ORCID: https://orcid.org/0000-0002-7727-4814)
Institutions
- Shanghai University (CN)
- Shanghai International Studies University (CN)
- East China University of Science and Technology (CN)
Publication Details
- Journal
- Journal of Advanced Research
- Published
- 2025-04-22
- DOI
- https://doi.org/10.1016/j.jare.2025.04.031
- Primary Topic
- CRISPR and Genetic Engineering
- Type
- article
- Field-Weighted Citation Impact
- 0.00