Molecular detection of Bartonella spp. DNA in dogs with hemangiosarcoma
Due to decreased DNA amplification efficiency, FFPE scrolls should not be used for the detection of Bartonella infection in spleen samples from dogs with HSA. PCR testing of fresh frozen tissues substantially improves the detection of Bartonella spp. infection. If fresh frozen tissues are not available, formalin-fixed tissues should be tested with digital PCR to enhance Bartonella DNA detection.
Authors
- Ricardo G. Maggi (ORCID: https://orcid.org/0000-0001-7428-0138)
- Daniel P. Regan (ORCID: https://orcid.org/0000-0002-3148-1332)
- Erin Lashnits (ORCID: https://orcid.org/0000-0003-0949-5698)
- Keith E. Linder (ORCID: https://orcid.org/0000-0002-2970-2992)
- Cynthia Robveille (ORCID: https://orcid.org/0000-0002-2264-307X)
- Taryn Donovan (ORCID: https://orcid.org/0000-0001-5740-9550)
- Kevin Woolard (ORCID: https://orcid.org/0000-0003-3588-9359)
- Edward B. Breitschwerdt (ORCID: https://orcid.org/0000-0002-3506-0279)
Institutions
- University of Wisconsin–Madison (US)
- North Carolina State University (US)
- The Schwarzman Animal Medical Center (US)
- University of California, Davis (US)
- Colorado State University (US)
Publication Details
- Journal
- PLoS ONE
- Published
- 2025-04-22
- DOI
- https://doi.org/10.1371/journal.pone.0321806
- Citations
- 1
- Primary Topic
- Bartonella species infections research
- Type
- article
- Field-Weighted Citation Impact
- 2.05
Funders
- American Kennel Club Canine Health Foundation