Interactions between dissolved organic matter composition and microbial communities in runoff from biocrust-colonized slopes on the Loess Plateau of China

The Zhifanggou watershed of the Loess Plateau, China. Using rainfall simulation experiments coupled with high-throughput sequencing and fourier transform ion cyclotron resonance mass spectrometry (FT-ICR MS), we revealed the linkages between DOM molecular characteristics and bacterial/fungal communities in runoff from slopes colonized by three typical biocrusts (cyanobacterial, cyanobacterial-moss, and moss crusts), with bare slope as control. Biocrust succession significantly increased DOM molecular diversity by 12–24 % compared to bare slope, while maintaining stable microbial diversity. The bacterial communities in runoff were dominated by Proteobacteria and Actinobacteriota (61–79 %), while Ascomycota (60–94 %) prevailed fungal communities. The DOM molecular composition in runoff was closely associated with bacterial and fungal communities and the biocrusts characteristics within runoff plots. The compositional differences of all compound classes or elemental combinations in runoff generally decreased with biocrust succession. Besides, the positive Spearman correlation between bacteria and DOM in runoff predominantly distributed in higher O/C and lower H/C molecular families, while vice versa for the fungi. Meanwhile, bipartite network analysis further demonstrated that both positive and negative interactions were closely associated to molecular characteristics. When the H/C or O/C ratios of DOM molecules were more similar, their correlations with microbial taxa showed greater similarity. Overall, our findings offer insights into DOM-microbe co-transport mechanisms at soil-aquatic interfaces of semiarid ecosystems. • DOM compositional differences in runoff decreased with the succession of biocrusts. • The link between DOM and microbial taxa highly affected by the biocrust type. • DOM-microbial interactions depend highly on the molecular features of DOM. • Microbial decomposition process was more specialized than the production process.