Antifungal Volatile Organic Compounds from Talaromyces purpureogenus CEF642N: Insights from One Strain Many Compounds (OSMAC) Strategy for Controlling Verticillium dahliae in Cotton
Verticillium wilt is a devastating soil-borne disease that significantly impacts cotton production, necessitating the development the effective biofumigants for its control. In this study, the inhibitory effect of total volatile organic compounds (VOCs) produced by <i>Talaromyces purpureogenus</i> CEF642<sup>N</sup> against <i>Verticillium dahliae</i> were evaluated using the one strain many compounds (OSMAC) strategy and analyzed through volatile metabolome. CEF642<sup>N</sup> was found to produce two primary VOCs, 3-octanol and 2-octenal, (E)-, both of which demonstrated significant antifungal activity. Transcriptome analysis of mycelium grown on various solid media revealed notable differences in the expression of genes associated with arachidonic acid metabolism, lipoxygenase (LOX), and lytic enzymes. These findings provide a foundation for future research aimed at identifying key genes involved in the eight-carbon volatile biosynthetic pathway.
Authors
- Hongjie Feng (ORCID: https://orcid.org/0000-0001-8039-3227)
- Peng Li
- Heqin Zhu (ORCID: https://orcid.org/0000-0002-4889-5320)
- Zili Feng (ORCID: https://orcid.org/0000-0002-5643-5420)
- Jinglong Zhou (ORCID: https://orcid.org/0000-0002-3813-0201)
- Yalin Zhang (ORCID: https://orcid.org/0000-0002-3296-0239)
- Lihong Zhao (ORCID: https://orcid.org/0000-0002-6116-8769)
- Feng Wei
Institutions
- Cotton Research Institute (CN)
- Chinese Academy of Agricultural Sciences (CN)
Publication Details
- Journal
- Journal of Fungi
- Published
- 2025-04-22
- DOI
- https://doi.org/10.3390/jof11050332
- Primary Topic
- Plant-Microbe Interactions and Immunity
- Type
- article
- Field-Weighted Citation Impact
- 0.00
Funders
- Chinese Academy of Agricultural Sciences
- Agricultural Science and Technology Innovation Program