Antioxidant Properties [Singlet Oxygen Scavenging and Nitric Oxide Radical Scavenging] of Thymus vulgaris and Piper nigrum and Screening of Its Main Constitutes Using Fourier Transform Infrared Spectroscopy Technique

Several types of medical substances derive from natural origins. The present pharmaceutical market contains fifty percent of drugs which derive from plants or utilize natural substances as their active components. Natural approaches became more widespread through the urgent need for new medical solutions to health problems. The selectivity levels proved superior when natural chemicals were used to target antibiotic-resistant bacteria and cancer cells when compared to artificial chemicals. The research investigated two aspects of antioxidant properties that included Singlet Oxygen Scavenging and Nitric Oxide Radical Scavenging through examination of Thymus vulgaris and Piper nigrum. Screening of Its Main Constitutes Using Fourier Transform Infrared Spectroscopy Technique. FT-IR peak values of solid analysis of Thymus vulgaris [669.30 and 684.73 Strong, C-Cl, alkyl halides], [827.46, 873.75, 927.76 Strong, =C–H, Alkenes], [1010.70, 1236.37, 1313.52 Strong, C-F, alkyl halides], [1417.68, Medium, C=C, Stretch, Aromatic], [1604.77, Bending, N-H, Stretch, Amide], [2918.30, Strong, C-H, Stretch, Alkane], [3269.34, Bending, N-H, Stretch, Amide]. FT-IR peak values of solid analysis of Piper nigrum [667.37, Strong, C-Cl, alkyl halides], [873.75, 921.97, Strong, =C–H, Alkenes], [1026.13, 1026.13, 1139.93, 1234.44, 1317.38, 1379.10 Strong, C-F, alkyl halides], [1415.75, 1519.91, 1598.99 Medium, C=C, Aromatic], [1740.72, Strong, C=O, Aldehyde], [2852.72, Strong, C-H, Alkane]. Antioxidant Properties [Singlet Radical Scavenging] of Crude (methanolic extract), Ethanol, Water and Lipoic acid (standard) of Thymus vulgaris recorded 62.05 ± 4.49, 48.17 ± 2.99, 59.08 ± 3.97 and 39.00 ± 1.26 respectively and recorded 58.96 ± 4.05, 45.00 ± 2.08, 55.97 ± 3.00 and 34.81 ± 1.09 for Piper nigrum while Antioxidant activity [Nitric oxide radical scavenging] of Crude (methanolic extract), Ethanol, Water and curcumin (standard) of Thymus vulgaris recorded 41.97 ± 2.04, 37.16 ± 2.03, 54.00 ± 2.99 and 89.17 ± 7.22 respectively and recorded 45.00 ± 2.19, 39.08 ± 2.14, 60.27 ± 3.89 and 96.13 ± 7.81 respectively for Piper nigrum. Thorough scientific studies should explore the medical and pharmaceutical characteristics of Thymus vulgaris and Piper nigrum since the plant demonstrates multiple treatment applications in medicine.