Optimization of Production Parameters and Partial Purification of a Klebsiella pneumoniae Lipase Isolated from Freshly Produced Palm Oil

Lipases are important hydrolytic enzymes with numerous industrial applications and economic impact. Lipase producing microorganisms were isolated from freshly produced palm oil produced in Akure, Nigeria. The microbes were assessed for lipase production potential using submerged fermentation. The microorganism with the best production potential was used for optimization studies. The microorganism was identified as Klebsiella pneumoniae. Optimal lipase production was obtained at 48 hours incubation period with fructose as best carbon source and yeast extract as best nitrogen source at pH 9. Klebsiella pneumoniae lipase production was optimal at 40 °C. High lipase activity in fresh palm oil increases the production of free fatty acids. Findings from this study indicates that the Klebsiella pneumoniae lipase could be of biotechnological significance in various industries.