Investigation Effects of Some Plant Hormones and Vitamins on Growth in Kanamycin-Resistant Tomato Varieties

Tomato is a widely consumed vegetable around the world and has a significant economic impact on agricultural production. However, various challenges in current agricultural practices can affect tomato production. To overcome these challenges and create a sustainable agricultural model, research focusing on tomato plant tissue culture is gaining importance. In this study, the neomycin phosphotransferase (NPTII) reporter gene was transferred to the Lycopersicon esculentum cLN1558A line through A. tumefaciens LBA4404. The effects of phenolic compounds and growth regulators used in tomato tissue culture were investigated. A balanced concentration of 1 mg ml-1 6-benzyladenine (BA) and 1 mg ml-1 gibberellic acid (GA3) was used for optimal shoot regeneration. For optimal root regeneration, 1 mg ml-1 indole-3-acetic acid (IAA) concentration was used. 200 μM acetosyringone was added to the culture medium to accelerate A. tumefaciens infection. As a result, it was confirmed that kanamycin resistance reached 50% in transgenic tomato shoots containing the NPTII gene. The efficiency of the phenolic compounds and growth regulators used increased by 12%.