Review on Microfluidic Surface Plasmon Resonance-Based Chip for Monitoring Biomechanisms

One important analytical technique for examining biomolecular interactions in real time is surface plasmon resonance, or SPR. SPR offers label-free, non-destructive monitoring of molecule binding events by detecting changes in the refractive index at the sensor surface. SPR sensors for tracking biological functions have been integrated with microfluidic technology to boost sensitivity and throughput in biosensing applications. It begins by summarizing the fundamental concepts of SPR and how they relate to biosensing. Then, as SPR is combined with microfluidic systems, the benefits and challenges of this technology are emphasized. The rest of the paper discusses the many uses of microfluidic SPR platforms, including cell-based assays, DNA hybridization, and the study of protein-protein interactions. This review article offers a thorough examination of SPR devices based on microfluidic chips for tracking biological processes. The basic ideas of SPR and their connection to biosensing are covered first. SPR's advantages and disadvantages are then highlighted as it is used with microfluidic devices. The remainder of the study explores the wide range of applications for microfluidic SPR platforms, such as DNA hybridization, cell-based investigations, and the analysis of protein-protein interactions.