Characterization of Cd and As accumulation and subcellular distribution in different varieties of perennial ryegrasses

Low Cd accumulation variety mainly resisted heavy metal through bound more Cd and As to cell wall resulting in cell wall thicken. High-Cd accumulation variety DPB stored Cd and As in the soluble fraction (vacuole ), and enhanced activity of the transporter protein HMA. This study elucidates the relationship and role of key transporter proteins of high/low accumulating perennial ryegrass with cellular Cd/As detoxification modes such as cell wall barrier defence and vesicle compartmentalisation, and provides a theoretical basis for differential detoxification strategies for species with different accumulating characteristics.