Abstract 5744: Design and synthesis of novel sulfonamide-based simple and vital aromatic amines as anticancer agents

Abstract Cancer remains a leading cause of mortality worldwide, with an increasing incidence driven by the complexity of tumor biology, drug resistance, and limited efficacy of current treatment options. Targeted therapy with small molecules has emerged as a promising approach due to its ability to selectively inhibit cancer cell growth while minimizing off-target effects. In this study, a series of derivatives synthesized from 3-formyl-4-hydroxy benzene sulfonyl chloride (FHS) were evaluated for their cytotoxic effects on various cancer cell lines. The objective was to identify compounds with potent anticancer activity and favorable selectivity profiles for further development. The synthesis process began with FHS as the starting material, subjected to a series of chemical reactions to generate a library of compounds labeled C3 through C10. These compounds were characterized using a combination of analytical techniques, including melting point determination, Proton Nuclear Magnetic Resonance (1H NMR), Carbon-13 Nuclear Magnetic Resonance (13C NMR), and Infrared (IR) spectroscopy to confirm their structural integrity. Cytotoxic effects were evaluated using Sulforhodamine B (SRB) assay. The assay was applied to an extensive panel of cancer cell lines, categorized into such as colorectal cancer (HT-29, MC38, CT26and COLO 205), hepatic (C3a and PLC/PRF), pancreatic (Suit-2, MiaPaca2, As-PC1 and Panc 02.03, CI66), breast (MDA-MB-468, MDA-MB-231, BT20, MCF7, 4T1, 4T1 paclitaxel resistant, JIMT-1, ZR-75-1, E0771, T47D, and HCC1806) and ovarian (OVCAR-5) cell lines. Normal breast epithelial cell line MCF10a was included as a control. Based on the SRB assay the 7 compounds exhibited variable but significant cytotoxicity in various cancer models. Further experiments are in progress to validate the most effective compounds in selected cancer models. Citation Format: Mohamed A. Eltokhy, Eman A. Fayed, Moustafa S. Abusaif, Ahmed Rageb, Yousry A. Ammar, Sanjay K. Srivastava. Design and synthesis of novel sulfonamide-based simple and vital aromatic amines as anticancer agents [abstract]. In: Proceedings of the American Association for Cancer Research Annual Meeting 2025; Part 1 (Regular Abstracts); 2025 Apr 25-30; Chicago, IL. Philadelphia (PA): AACR; Cancer Res 2025;85(8_Suppl_1):Abstract nr 5744.