Abstract 2321: Longitudinal evidence of healthy lifestyles slowing biological aging and reducing cancer risk

Abstract Background: Aging is an important risk factor of cancer, and a healthy lifestyle is one possible measure to delay aging. Limited research has investigated the relationship between a healthy lifestyle, biological aging, and cancer incidence. Methods: This prospective study enrolled 122, 023 individuals from the Taiwan MJ cohort. The multivariable logistic regression model was utilized to examine the associations between the healthy lifestyle scores (HLS, integrating never or quitted smoking, non-harmful drinking, active physical activity, healthy body fat percentage, and healthy diet) and biological aging acceleration alteration, which was measured by changes in multi-dimensional aging measure acceleration (ΔMDAgeAccel). The Cox regression model was used to evaluated the joint association of HLS and ΔMDAgeAccel with cancer risk. Results: Compared to individuals with HLS of 0, increasing HLS was associated with decreased odds of MDAgeAccel increase, with the adjusted odds ratio (aOR) of 0.684 (95% CI 0.557 to 0.841), 0.589 (0.483 to 0.718), 0.500 (0.411 to 0.610), 0.426 (0.349 to 0.519), and 0.379 (0.310 to 0.464) for HLS of 1, 2, 3, 4, and 5, respectively. These results remained consistent across stratifications by baseline aging acceleration status, age group, and sex. Individuals with accelerated aging at baseline and higher HLS exhibited lower odds ratio of persistent aging acceleration. The hazard ratio (HR) for cancer incidence was 0.774 (0.665 to 0.900) for individuals with a higher HLS and decreased MDAgeAccel, 0.932 (0.779 to 1.116) for individuals with a higher HLS and increased MDAgeAccel, compared with those with a lower HLS and increased MDAgeAccel. Conclusions: Adherence to healthy lifestyle may lower aging acceleration, and reducing cancer risk. Citation Format: Junlin Jia, Chi Pang Wen, Huakang Tu, Junlong Pan, Wanzhu Lu, Min Yang, Andi Xu, Sicong Wang, Wenyuan Li, Xifeng Wu. Longitudinal evidence of healthy lifestyles slowing biological aging and reducing cancer risk [abstract]. In: Proceedings of the American Association for Cancer Research Annual Meeting 2025; Part 1 (Regular Abstracts); 2025 Apr 25-30; Chicago, IL. Philadelphia (PA): AACR; Cancer Res 2025;85(8_Suppl_1):Abstract nr 2321.