USING CuO NPs TO INCREASE SOME TERPENES ON LAVANDULA ANGUSTIFOLIA IN VITRO

This article was designed to check the ability to increase some terpenes active compounds production in Lavandula angustifoliaIn In Vitro plantlets through supplemented media with vary concentrations of Nano Copper oxide.This project was conducted at plant tissueculture lab.belongs to the biotechnology facuality -Al Nahrain University during 2023 and 2024.The experimental design used to analays data is factorial CRD using ten replicates.Sodium hypochlorite concentration used in contamination test was (1, 2, 3%) represented treatment duartion time (5, 15, 20min) represented.Meanwhile Copper oxide nanoparticles (0, 1, 2, 3mg.l) was added to the MS media.Results indicated the highest contamination rate reduction in the Lavender explant recorded in (2% sodium hypochlorite combined with 20 min).The results also clarified shoots numbers was significantly haighest at 30 mg. lCuONPs, shoot length also significantly increased within the10 mg.l then decreased in both 20 and 30 mg. lCuONPs, in seedlings fresh weight was significantly the highest in 20 mg.l CuONPs, based on the results seedlings dry weight was significantly dropped in 30 mg. l CuONPs concentration.Also, HPLC device was used to analyze terpenes compounds as aromatic compounds, finally terpenoids, isoprenoids estimated values were increased significantly in both 20 and 30 mg. lCuONPs, concentrations compared to control.