Abstract 5168: Mouse models accelerating cancer research at The Jackson Laboratory

Abstract The Jackson Laboratory (JAX) provides genetically defined mutant and transgenic mice to support the scientific research community. Available are mouse models for specific cancers, xenograft recipients, immunodeficient platforms for PDX studies and multi-purpose tool strains having CRISPR utility, conditional/inducible expression (Cre-lox, FLP-frt, Tet-On/-Off), optogenetic function and calcium-sensing technologies. This poster will highlight newly developed models designed for cancer research. The portfolio of strains useful for transplantation and humanized mouse research continues to expand and complement the NSG (NOD scid IL2rγc-/-) model as premier tools for engraftment with a wide range of malignant or non-malignant human or mouse tissues. Novel fluorescent strains include Gene Editing Reporters for visualizing homology-directed repair (HDR) and non-homologous end joining (NHEJ) repair events, as well as several CRISPR Editing, Bxb1 Integrase Editing, Base Editor, Prime Editor and DNA repair pathway tools. Furthermore, new strains enable researchers to achieve extensive barcode diversity within targeted cell populations. Models utilizing the auxin-inducible degron system allow degradation of tagged proteins following administration of 5-Ph-IAA. In addition to cryopreserving each strain, JAX employs a genetic quality control program that confirms mutation identity and genetic background, and screens for unwanted alleles (such as stray GFP, Cre, neo, etc.). Cancer researchers can query the collection of innovative mouse models using the JAX Mouse Search site (mice.jax.org). This resource includes models created by many generous donating institutions. Researchers are encouraged to donate their mouse lines via a very short Strain Submission form (jax.org/donate-a-mouse). Please also visit the JAX Oncology webpage for cancer research related strains and resources (jax.org/jax-mice-and-services/solutions-by-therapeutic-area/oncology). JAX is supported by the NIH, The Howard Hughes Medical Institute and other private charitable foundations. Citation Format: Jason Beckwith, Jen Merriam, Darcy Pomerleau, Crystal Davis, Jennifer Kelmenson, Hiroaki Onda. Mouse models accelerating cancer research at The Jackson Laboratory [abstract]. In: Proceedings of the American Association for Cancer Research Annual Meeting 2025; Part 1 (Regular Abstracts); 2025 Apr 25-30; Chicago, IL. Philadelphia (PA): AACR; Cancer Res 2025;85(8_Suppl_1):Abstract nr 5168.