Abstract 1774: A novel role of SNORA71D in the regulation of proteasome inhibitor induced apoptosis

Abstract Small nucleolar RNAs (snoRNA) are 60-300 nucleotide long RNAs that guide modification of rRNAs during ribosome biogenesis. Many of them have non-canonical functions, such as regulation of splicing of specific mRNAs. We have made a serendipitous discovery that treatment of cells with proteasome inhibitors (PIs) induces expression of a poorly characterized snoRNA, SNORA71D. Two other agents that induce production of misfolded proteins, tunicamycin and thapsigargin, also induced SNORA71D, but DNA-damaging agent doxorubicin did not. Knocking SNORA71D down reduced PI-induced apoptosis. The mechanisms of SNORA71D involvement in this process is under investigation. These findings indicate a novel and unexpected role of SNORA71D in cellular response to proteotoxic stress. Citation Format: Alexei F. Kisselev, Olasubomi A. Akintola, Addison M. Wilson. A novel role of SNORA71D in the regulation of proteasome inhibitor induced apoptosis [abstract]. In: Proceedings of the American Association for Cancer Research Annual Meeting 2025; Part 1 (Regular Abstracts); 2025 Apr 25-30; Chicago, IL. Philadelphia (PA): AACR; Cancer Res 2025;85(8_Suppl_1):Abstract nr 1774.