Hydrolyzed Marine Collagen Loaded Nanoemulsion : Characteristics and Irritation Study Based on Vegetable Oils Combination

Abstract The exploration of natural sources for collagen to combat skin aging has gained significant attention, particularly through marine sources known as marine collagen. This study focuses on Hydrolyzed Marine Collagen, which, due to its hydrophilic nature, faces challenges in penetration. The nanoemulsion delivery system offers the advantage of overcoming Hydrolyzed marine collagen limitations. The study examined four formulations using different fixed oils — Virgin Coconut Oil (VCO), Olive Oil, and Jojoba Oil—combined with the surfactant PEG-7 Glyceryl Cocoate. The ratios of oil phase to surfactant to water phase were as follows: FN 1 (4:40:56), FN 2 (6:45:49), FN 3 (10:65:25), and FN 4 (15:70:15). All formulations resulted in a clear yellow consistency and were oil-in-water (o/w) emulsions. pH testing revealed that F4 had the highest pH at 6.72 ± 0.03, while the particle size analysis showed F1 had the smallest particles at 20.24 ± 1.89 nm. All formulations had a polydispersity index of less than 1, indicating uniformity. Irritation tests using HET-CAM indicated no irritation from any of the formulations. The study concludes that the ratios of oils, surfactants, and water in the nanoemulsions influence their characteristics while ensuring no irritation.