Abstract 3167: Longer and brighter live cell bioluminescence prosubstrates

Abstract Bioluminescence prosubstrates are chemically protected bioluminescence substrates which, upon introduction to cells, can gradually and slowly release free substrate for generating luminescent signals in cellular assays. Prosubstrates can generate steady signals that last for several hours or days, enabling live-cell, kineticassays. Noticeable examples of bioluminescence prosubstrates include Nano-Glo Endurazine and Vivazine Live Cell Substrates. Although these prosubstrates have been serving the scientific community for years, they still have drawbacks, including reduced brightness, sensitivity, and signal duration. Herein, we describe a new generation of live cell bioluminescence prosubstrates that can overcome the drawbacks of current prosubstrates. Citation Format: Chao Gao, Brock Binkowski, Hui Wang, Wenhui Zhou. Longer and brighter live cell bioluminescence prosubstrates [abstract]. In: Proceedings of the American Association for Cancer Research Annual Meeting 2025; Part 1 (Regular Abstracts); 2025 Apr 25-30; Chicago, IL. Philadelphia (PA): AACR; Cancer Res 2025;85(8_Suppl_1):Abstract nr 3167.