Abstract 6321: Vialinin-A, an antioxidant isolated from edible mushroom <i>thelephora vialis</i>, inhibits colorectal cancer growth

Colorectal cancer (CRC) is the third leading cause of cancer-related deaths in the United States. While CRC screening typically begins at age 45-50, its incidence is rising in younger populations, highlighting the need for diversified treatment options. Genetic predisposition, oxidative stress, and inflammation are recognized as major contributors to the pathogenesis of this disease. Current treatments, such as chemotherapy and surgical resection, often lead to unwanted side effects and risks, including potential cardiotoxicity from chemotherapeutics like anthracyclines. Vialinin-A, a compound isolated from the edible Chinese mushroom Thelephora Vialis, has demonstrated antioxidant and anti-inflammatory properties. However, its anti-carcinogenic mechanisms are not well known. Human colon cancer cells such as SW480 and CaCO-2 were treated with varying concentrations of Vialinin-A for 24 and 48 h. Cell viability was determined by MTT assay. Apoptosis was evaluated through the activation of caspase-3 and the use of a human apoptosis array to analyze the expression of key apoptotic markers. Additionally, a multiplex array was used to identify oncogenic, inflammatory, and apoptotic markers in treated cells. Next, we plan to use nude mice xenografts to examine the chemopreventive role of Vialinin-A in vivo. So far, our results indicate that Vialinin-A inhibits CRC cell growth in a dose-dependent manner and suppresses the generation of reactive oxygen species (ROS). Vialinin-A activates caspase-3 in growth factor-treated cancer cells. Furthermore, Vialinin-A modulates the expression of various apoptotic and inflammatory markers in CRC cells, supporting its potential role in anti-carcinogenic pathways. Ongoing experiments aim to further elucidate the molecular mechanisms through which Vialinin-A inhibits CRC growth. Citation Format: Divya Lahori, Dallin Thornton, Kota V. Ramana. Vialinin-A, an antioxidant isolated from edible mushroom thelephora vialis, inhibits colorectal cancer growth [abstract]. In: Proceedings of the American Association for Cancer Research Annual Meeting 2025; Part 1 (Regular Abstracts); 2025 Apr 25-30; Chicago, IL. Philadelphia (PA): AACR; Cancer Res 2025;85(8_Suppl_1):Abstract nr 6321.