Research progress in methods for glycan sulfation

The sulfation modification of glycans holds immense significance in living organisms, playing a pivotal role in numerous physiological and pathological processes.In-depth studies into glycan sulfation methods are crucial for unraveling the biological functions of glycans and advancing the development of carbohydrate-based pharmaceuticals and related biomaterials.This review comprehensively summerized the research advancements in glycan sulfation methodologies, encompassing traditional chemical methods, enzymatic methods, chemo-enzymatic methods, and sulfation methods augmented by emerging technologies.It meticulously delineates the principles, distinctive features, illustrative applications, and inherent challenges associated with each method, while also forecasting the future trajectory of development in this field.