Histology-resolved proteomics reveals distinct tumor and stromal profiles in low- and high-grade prostate cancer
Proteomic alterations between Gleason 6 versus Gleason 9 were exclusive to the disease microenvironment, observed in both the tumor epithelium and tumor-involved stroma. Further, the molecular alterations measured in the tumor-involved stroma from Gleason 9 cases relative to the benign stroma have unique significance in disease aggressiveness, development, and/or progression. Our data provide supportive evidence of a need for further investigations into targeting stromal reservoirs of cholesterol and/or deoxynucleoside triphosphates in PCa tumors and further highlight the necessity for independent examination of the TME epithelial and stromal compartments.
Authors
- Nicholas W. Bateman (ORCID: https://orcid.org/0000-0002-4425-9511)
- Tamara L. Lotan (ORCID: https://orcid.org/0000-0002-0494-9067)
- Waleed Barakat (ORCID: https://orcid.org/0000-0003-2056-3559)
- Jonathan D. Ogata (ORCID: https://orcid.org/0000-0002-8082-7395)
- Allison L. Hunt (ORCID: https://orcid.org/0000-0002-2223-7944)
- Tamara Abulez (ORCID: https://orcid.org/0000-0001-5325-5064)
- Sasha C. Makohon‐Moore (ORCID: https://orcid.org/0000-0002-5915-283X)
- Brian L. Hood (ORCID: https://orcid.org/0000-0002-2398-487X)
- Thomas P. Conrads (ORCID: https://orcid.org/0000-0003-4742-3281)
- Kenneth J. Pienta (ORCID: https://orcid.org/0000-0002-4138-2186)
- Donald L. Trump
- Katlin N. Wilson
- Haresh Mani
- Kelly A. Conrads
Institutions
- Inova Health System (US)
- Henry M. Jackson Foundation (US)
- Johns Hopkins University (US)
- Uniformed Services University of the Health Sciences (US)
- Johns Hopkins Medicine (US)
- Walter Reed National Military Medical Center (US)
- Inova Fairfax Hospital (US)
- Sidney Kimmel Comprehensive Cancer Center (US)
Publication Details
- Journal
- Clinical Proteomics
- Published
- 2025-04-20
- DOI
- https://doi.org/10.1186/s12014-025-09534-8
- Citations
- 1
- Primary Topic
- Prostate Cancer Treatment and Research
- Type
- article
- Field-Weighted Citation Impact
- 1.52