Improvements in real-time PCR methodology for detection of infectious bursal disease virus

The aim of the study was to modify the PCR-method for real-time detection of infectious bursal disease (IBD) virus. The samples of synthetic oligonucleotide primers to the highly conserved overlapping region VP5/VP2 of segment A of infectious bursal disease virus were selected. The primers used in the study have sensitivity and specifi city and can be used in diagnostic studies to detect IBD virus RNA.

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Publication Details

Journal
Poultry and Chicken Products
Published
2025-01-01
DOI
https://doi.org/10.30975/2073-4999-2025-27-1-58-61
Primary Topic
Virology and Viral Diseases
Type
article
Field-Weighted Citation Impact
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article

Improvements in real-time PCR methodology for detection of infectious bursal disease virus

Konstantin Dmitriev, A.N. Seminа
Poultry and Chicken Products
Virology and Viral Diseases
article

Improvements in real-time PCR methodology for detection of infectious bursal disease virus

Konstantin Dmitriev, A.N. Seminа
article en

Abstract

The aim of the study was to modify the PCR-method for real-time detection of infectious bursal disease (IBD) virus. The samples of synthetic oligonucleotide primers to the highly conserved overlapping region VP5/VP2 of segment A of infectious bursal disease virus were selected. The primers used in the study have sensitivity and specifi city and can be used in diagnostic studies to detect IBD virus RNA.

Poultry and Chicken ProductsVol. 27(1)
Openalex Percentile: Top 88%
Virology and Viral Diseases
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Improvements in real-time PCR methodology for detection of infectious bursal disease virus — Konstantin Dmitriev, A.N. Seminа · Poultry and Chicken Products (2025) | TGRS Research Map | TGRS