Validating the Crude Oil Degrading Ability of Bacillus Subtilis Isolated From Crude Oil Contaminated Soil

Oil spills have caused significant ecological issues. The process of hydrocarbon bioremediation relies on the microbial population’s capacity to proliferate within these substrates and breakdown in to harmless byproducts Bioremediation represents a viable and auspicious alternative to conventional cleaning methods. Soil microorganisms possess the ability to mineralize the majority of the organic compounds. Hence, the current study mainly focus on the crude oil degrading bacterial isolation, degradation, molecular analysis and optimization of bacterial isolates present in crude oil contaminated soils from automobile service centre at Namakkal, Tamil Nadu. Through the process of enrichment and screening, bacteria that possess the capability to degrade major crude oil were selected and subsequently subjected to 16s rRNA sequencing method to determine their identity. In order to optimize the cultural conditions a range of factors includes pH, temperature, crude oil concentration and nitrogen source were carried out. Totally thirty six organisms were isolated from the enrichment method. Growth of these isolates was examined and the isolate named PS9 showed higher capacity (56%) for the utilization of crude oil. GC-MS study confirmed an observable crude oil degradation of the samples treated with PS9 when compared to control crude oil. In the analysis of 16s rRNA sequencing data, the isolated strain belong to the species Bacillus subtilis. Analysis of variance was used to ensure statistical accuracy, low p<0.0001 was obtained. Based on these studies the above bacterial strain can be used in biodegradation.