Ultrastructural Changes in Final Instar Larvae of Papilio polytes (Lepidoptera: Papilionidae) Lead to Differences in Epidermal Spreading of Water and Adjuvants

<i>Papilio polytes</i> is a cosmopolitan Lepidoptera species of controversial use and management. It remained unclear how its epidermal ultrastructure changes during development and how this affects its wetting properties in relation to water and pesticide adjuvants. In this study, the epidermis of <i>P. polytes</i> was systematically examined at the important feeding stage (from 3rd to 5th instar). Its ultrastructure was quantitatively observed by scanning electron microscopy. Its wetting properties towards the three main types of adjuvants and water were evaluated by contact angle. The chemical functional group differences between different instars and different adjuvant treatments were analyzed by mid-infrared spectroscopy. The correlation between the ultrastructural deformation and variations in wetting properties was verified by simulation tests. It was found that the complication of the epidermal structure was the leading factor for the significant increase in hydrophobicity during development. Cationic adjuvants had the best infiltrating effect on complex epidermal structures and organosilicon adjuvants had the best infiltrating effect on simple epidermal structures. The results provide data for biomimetic design for different wetting properties and suggest the feasibility and advantages of selecting pesticide adjuvants based on developmental changes in the structural characteristics of the insect epidermis.