STUDY OF LASER COMPOUND EFFICIENCY IN COMPLEX WITH BIOLOGICAL COMPOSITIONS FOR SKIN DEFECT REPAIR IN FLAP PLASTY IN ACUTE EXPERIMENT

Abstract. In an experimental surgical study, laser technologies and various types of biological compounds effectiveness, collagen-containing and collagen-free, in closing a skin defect with subsequent excision and closure with counter flaps using the A.A. Limberg surgeon method were studied. The research was performed on 27 male Wistar laboratory rats weighing 200-250 g, which were divided into 3 groups of 9 species each to obtain representative results: 1 - control group with flap plastic surgery with counter flaps using interrupted sutures at intervals of 5 mm; 2 - experimental group using laser technologies and biological composition 1 (BC-1) containing serum albumin 25 wt.%, collagen 8 wt.%, indocyanine green 0.1 wt.%, single-wall carbon nanotubes 0.1 wt.%, water); 3 - experimental group using laser technologies and biological composition 2 (BC-2) containing serum albumin 25 wt.%, indocyanine green 0.1 wt.%, single-wall carbon nanotubes 0.1 wt.%, water. On days 7, 14, 21, laboratory animals were taken out of the experiment, 3 individuals at random, under inhalation anesthesia with isoflurane (USA) in a specialized CO2 chamber. The wound healing time, the thickness of the formed scar connection, the tensile strength of the suture, the morphological structure of the connection using hematoxylin and eosin staining (histological sections were studied in an Olympus-cx312 binocular microscope), and its cosmetic characteristics according to the international Scar scale were assessed. The use of BS-2, containing serum albumin 25 wt.%, indocyanine green 0.1 wt.%, single-wall carbon nanotubes 0.1 wt.%, water, under laser exposure is a reliable method for connecting the edges of a wound defect in flap plastic surgery, not inferior in strength to a nodal suture on the 21st day, reduces the time of primary scar formation to 8.1 ± 0.7, and has optimal cosmetic characteristics.