Comparative study of Monoiodoacetate (MIA) and Papain chemical model of osteoarthritis in Hartley strain guinea pigs

The present study was carried out to optimize the procedure for creation of chemical model of osteoarthritis (OA) in guinea pigs. Eighteen (18) clinically healthy adult Hartley strain guinea pigs of either sex, weighing 500 to 600 g were used for creation of OA model divided into two groups A and B having 9 animals each. Intra-articular injection of 1 mg of monosodium iodoacetate (MIA) dissolved in 100 μl of normal saline was done through the infrapatellar ligament of the right knee in group A using a 27-gauge, 0.5-inch needle. Papain enzyme at dose rate of 10mg in 0.05M sodium acetate (pH 4.5) with enzymatic activity of 31 I.U/mg was injected intra-articularly in right knee joint. The left control knee was injected with 100 μl of physiologic saline in both the groups. In the present study intra-articular injection of 1 mg of monosodium iodoacetate was found better than papain for induction of osteoarthritis on the basis of clinical, radiological, histological and biochemical biomarker studies.