Exposure to sub-zero temperatures down to -11 °C does not impact packed red cells storage quality
European guidelines require packed red blood cells (pRBC) to be stored at 2-6 °C. However, negative temperature shifts can occur especially in prehospital transfusion. We investigated the impact of sub-zero temperature exposure on pRBC storage quality. At day 6 post donation (D6), three cohorts (14 pRBC) were put on a supercooled table for 10 h at either - 1 °C, -5 °C, and - 11 °C and compared to a control cohort. Hemolysis, pH and plasma biochemistry were evaluated weekly until D49. Storage-induced micro-erythrocytes (SMEs) were quantified as a surrogate marker for transfusion recovery. The primary endpoint was compliance with European storage standards at D42. The three sub-zero-exposed cohorts met standards at D42. Differences in hemolysis, pH, plasma biochemistry, or SMEs between exposed and control cohorts were non-statistically and/or non-clinically significant. Ten hours exposure to sub-zero temperatures down to -11 °C by conduction maintains storage quality of pRBCs, enabling a wiser risk assessment for potential transfusion use.
Authors
- Yann Daniel (ORCID: https://orcid.org/0000-0001-7068-9462)
- Olivier Javaudin (ORCID: https://orcid.org/0000-0002-8486-8573)
- Philippe Vest (ORCID: https://orcid.org/0009-0003-7878-7667)
- Daniel Jost (ORCID: https://orcid.org/0000-0002-6046-1234)
- Pascal Amireault (ORCID: https://orcid.org/0000-0002-5562-7586)
- Matthieu Heidet
- Manon Vavasseur (ORCID: https://orcid.org/0000-0003-2700-299X)
- Simon-Pierre Corcostegui
- Christophe Martinaud (ORCID: https://orcid.org/0000-0001-5439-4699)
- Paul Lely
- H. Desudde
- Michaël Dussiot (ORCID: https://orcid.org/0000-0002-6804-1621)
- Clément Derkenne (ORCID: https://orcid.org/0000-0001-8555-8000)
- Sandrine Pons (ORCID: https://orcid.org/0000-0001-7561-9869)
- Albane Miron de l'Espinay
- Angela Giannuzzo
- Elodie Ormes
Institutions
- Inserm (FR)
- Université Paris-Est Créteil (FR)
- Université Paris Cité (FR)
- Brigade des Sapeurs Pompiers de Paris (FR)
- Service de Santé des Armées (FR)
- Sorbonne Paris Cité (FR)
- Hôpital d'instruction des Armées Percy (FR)
- Institut des Maladies Génétiques Imagine (FR)
Publication Details
- Journal
- Scientific Reports
- Published
- 2025-04-19
- DOI
- https://doi.org/10.1038/s41598-025-98273-9
- Citations
- 1
- Primary Topic
- Erythrocyte Function and Pathophysiology
- Type
- article
- Field-Weighted Citation Impact
- 1.16
Funders
- Agence Nationale de la Recherche
- Université Paris-Est Créteil Val-de-Marne