New Approaches Based on Serial‐Block Face Electron Microscopy to Investigate the Peripheral Nervous System
Our findings indicate that automated uranyl-free staining can effectively compete with the traditional gold standard manual and uranyl-based staining methods, albeit with some limitations. Furthermore, high-quality SBF-SEM imaging is attainable, especially in peripheral nerves, using samples prepared via the standard TEM method, thereby facilitating the analysis of previously embedded samples even if they were not specifically prepared for 3D examination.
Authors
- P Marmiroli (ORCID: https://orcid.org/0000-0002-7590-7649)
- Vitālijs Borisovs (ORCID: https://orcid.org/0000-0002-5542-114X)
- M Bossi
- Laura Matino (ORCID: https://orcid.org/0000-0002-8130-2953)
- Guido Cavaletti (ORCID: https://orcid.org/0000-0003-4128-2406)
Institutions
- Azienda Ospedaliera San Gerardo (IT)
- Institute for Experimental Endocrinology and Oncology (IT)
- University of Milano-Bicocca (IT)
- University of Latvia (LV)
Publication Details
- Journal
- Journal of the Peripheral Nervous System
- Published
- 2025-04-19
- DOI
- https://doi.org/10.1111/jns.70019
- Primary Topic
- Molecular Biology Techniques and Applications
- Type
- article
- Field-Weighted Citation Impact
- 0.00
Funders
- Ministero dell'Università e della Ricerca