Development and Validation of a Stability Indicating HPLC Method for the Simultaneous Determination of Flunarizine and Domperidone in Tablet Dosage Form

This present work aimed to develop a simple, accurate, precise, and reproducible Stability Indicating RP-HPLC method for the simultaneous determination of Flunarizine and Domperidone Separation was achieved by chromatographic condition ACE Excel C18(250mm x 4.6mm, 5µm) column with the mobile phase composition 10 mM Potassium Dihydrogen Phosphate buffer: Acetonitrile: Methanol (40:30:30% v/v). The injection volume was set to 10µL. The detection was carried out at 220nm with a 1.0mL/min flow rate. Flunarizine and Domperidone were eluted with retention times of 3.4 min and 9.7 min respectively. Linearity was obtained over the concentration range of 25-75µg/mL for Flunarizine and 50-150µg/mL for Domperidone drug with for both correlation coefficients was 0.999. Accuracy was obtained between 98.89-101.84% and 98.46-101.55% for Flunarizine and Domperidone respectively. LOD was found to be 0.22μg/mL and 0.42μg/mL and LOQ were found to be 0.68μg/mL and 1.29μg/mL for Flunarizine and Domperidone respectively. The result revealed that the developed method is suitable for routine analysis of determining Flunarizine and Domperidone in a tablet dosage form.