Spectrophotometric Method Development and Validation for Simultaneous Estimation of Metformin Hydrochloride and Evogliptin Tartrate in Pharmaceutical Dosage Form

Simultaneous method has been developed for the estimation of Metformin hydrochloride and Evogliptin tartrate in pharmaceutical dosage form. The linearity was found to be in concentration range of 2 – 12μg/mL and 0.999 correlation coefficient at 233 nm and 0.998 at 267nm for Metformin hydrochloride. The linearity for Evogliptin tartrate was found to be in concentration range of 20 – 120μg/mL. The correlation coefficient was found to be 0.981 at 233nm and 0.999 at 267nm. The accuracy was found to be in the range of 98.02% - 100.74% and 99.09% - 101.5% for MFH and EGT respectively. The limit of detection (LOD) was found to be 0.13μg/mL and 2.39μg/mL for MFH and EGT respectively. The limit of quantification (LOQ) was found to be 0.386μg/mL and 7.253μg/mL for MFH and EGT respectively. The proposed method can be used for the determination of Metformin hydrochloride and Evogliptin tartrate in pharmaceutical dosage form. The simultaneous UV–Visible method was eco- friendly, simple, precise and accurate as per ICH guidelines. The proposed method can be used in quality control for routine analysis of Metformin hydrochloride and Evogliptin tartrate in the pharmaceutical dosage forms.