Generation of two induced pluripotent stem cell lines from breast cancer patients carrying BRCA1 mutations
Haploinsufficiency of BRCA1 increases the risk of various cancers due to its critical functions in cell cycle checkpoint regulation and DNA repair. This study generated and characterized two induced pluripotent stem cell (iPSC) lines derived from breast cancer patients with mutations in the BRCA1 gene (c.676del and c.5096G>A). Both lines exhibited typical iPSC morphology, karyotype, pluripotency marker expression, and trilineage differentiation capabilities. BRCA1-mutated iPSCs provide a valuable resource to investigate pathogenic mechanisms and develop personalized medicine.
Authors
- Viswanathan Mohan (ORCID: https://orcid.org/0000-0001-5038-6210)
- Christopher D. Yan
- Joseph C. Wu (ORCID: https://orcid.org/0000-0002-6068-8041)
- Arianne Caudal (ORCID: https://orcid.org/0000-0001-9836-9436)
- Walter G. Park (ORCID: https://orcid.org/0000-0001-8187-4188)
- Y.M. Chen (ORCID: https://orcid.org/0000-0001-5302-0433)
- Amira G Flores-Banuelos
Institutions
- Cardiovascular Institute of the South (US)
- Stanford Medicine (US)
- Stanford University (US)
Publication Details
- Journal
- Stem Cell Research
- Published
- 2025-04-18
- DOI
- https://doi.org/10.1016/j.scr.2025.103716
- Primary Topic
- CRISPR and Genetic Engineering
- Type
- article
- Field-Weighted Citation Impact
- 0.00
Funders
- Ministry of Science and Technology, Taiwan
- National Institutes of Health