Trichoderma spp. antagonistic relationship with Fusarium is linked to cotton Wilting

The experiment was carried out at Jamal Mohamed College (A), Triuchirappalli, Tamilnadu, during the Kharif season of 2022–2023, to assess the effectiveness of biological control methods based on Trichoderma species in the in vitro management of Fusarium species. Using a dual plate experiment, six rhizospheric Trichoderma species were isolates from six distinct cotton crop fields were examined in vitro for antagonistic activity against Fusarium wilt infections. Trichoderma species, isolate T4, was shown to successfully suppress the radial mycelial growth of the Fusarium species by 76.92% under in vitro conditions. The examined Fusarium species growth was hindered by the isolates of Trichoderma spp. The effects of abiotic stressors as pH, temperature, and NaCl on the growth of Trichoderma isolates were also investigated. The isolates with the highest resistance to abiotic stress were T1 and T4. The specific activity of chitinase produced by local isolates was investigated. The NIST and WILEY libraries have provided the results of the T4's ethyl acetate extracted product based on the compounds' retention time, molecular weight, molecular formula, and one chemical structure, which is demonstrated by GCMS analysis. Overall, isolate Tv4 showed the most promise in regulating the development of all Fusarium species isolates and demonstrating tolerance to abiotic stressors.