Evaluation of anti-oxidant, anti-inflammatory and toxicity potential of ethanolic extract of C.diurnum leaves

Plants contain several different phytochemicals which can act on various metabolic pathways of the human system at a time thus effectively treating several conditions at once. Main objective of this research was to identify and assess the anti-oxidant, anti-inflammatory and toxicity potential of Cestrum diurnum leaves extract. Phytochemical screening of plant extract was performed, followed by DPPH radical scavenging potential assay, FRAP assay and protein denaturation inhibition assay with both the plant extract and the standard drugs. Hemolytic toxicity was also determined to calculate the safe dosage of plant extract for consumption as a possible drug candidate against oxidative and inflammatory stress conditions. This study confirmed the presence of high quantities of total phenols and total flavonoids which in turn showed notable anti-oxidant potential but slightly reduced reduction potential. Higher protein denaturation inhibition rates indicated the efficiency of plant extract as an effective anti-inflammatory agent and nominal hemolytic activity at 100 μg/ml of plant extract. Further studies are required to ascertain the specific phytocompounds responsible for such activities and their potential applications as pharmacologically active compounds.