Development and construction of a novel Bacillus subtilis autoinducible extracellular expression system based on a LuxI/R device
To our knowledge, this is the first study to report enhanced autoinducible expression of the lux system in B. subtilis by engineering the sensing module promoter SP<sub>luxI</sub> sequence and the lux box copy number of the response module promoter RP<sub>luxIR6</sub>. This study further expands the application potential of the B. subtilis expression system in synthetic biology.
Authors
- Yanyan Zhao (ORCID: https://orcid.org/0000-0003-2302-1763)
- Yulu Fang
- Zemin Fang (ORCID: https://orcid.org/0000-0002-9853-5470)
- Dongbang Yao
- Keyi Wang (ORCID: https://orcid.org/0000-0002-6520-1562)
- Xin Zhao (ORCID: https://orcid.org/0000-0002-1078-7332)
- Yazhong Xiao (ORCID: https://orcid.org/0000-0003-2664-742X)
- Bin Wang (ORCID: https://orcid.org/0000-0002-0141-7305)
Institutions
- University of Science and Technology of China (CN)
- Anhui University (CN)
Publication Details
- Journal
- Microbial Cell Factories
- Published
- 2025-04-19
- DOI
- https://doi.org/10.1186/s12934-025-02719-8
- Citations
- 5
- Primary Topic
- Bacterial biofilms and quorum sensing
- Type
- article
- Field-Weighted Citation Impact
- 4.03
Funders
- National Natural Science Foundation of China
- Anhui University
- National Key Research and Development Program of China