1725-nm HOPE for segmentation-enabled quantitative photoacoustic microscopy of intrahepatic lipids

Photoacoustic microscopy (PAM) operating within the 1.7-μm absorption window holds great promise for the quantitative imaging of lipids in various biological tissues. Despite its potential, the effectiveness of lipid-based PAM has been limited by the performance of existing nanosecond laser sources at this wavelength. In this work, we introduce a 1725-nm hybrid optical parametric oscillator emitter (HOPE) characterized by a narrow bandwidth of 1.4 nm, an optical signal-to-noise ratio (OSNR) of approximately 34 dB, and a high spectral energy density of up to 480 nJ/nm. This advanced laser source significantly enhances the sensitivity of photoacoustic imaging, allowing for the detailed visualization of intrahepatic lipid distributions with an impressive maximal contrast ratio of 23.6:1. Additionally, through segmentation-based analysis of PAM images, we were able to determine steatosis levels that align with clinical assessments, thereby demonstrating the potential of our system for high-contrast, label-free lipid quantification. Our findings suggest that the proposed 1725-nm HOPE source could be a powerful tool for biomedical research and clinical diagnostics, offering a substantial improvement over current technologies in the accurate and non-invasive assessment of lipid accumulation in tissues.