Rotenone inhibited osteosarcoma metastasis by modulating ZO-2 expression and location via the ROS/Ca <sup>2+</sup> /AMPK pathway
Rotenone-induced ROS led to increased intracellular Ca<sup>2+</sup>, which promoted the EMT of OS cells through activation of AMPK and ZO-2 nuclear translocation. Inhibition of ROS production decreased intracellular Ca<sup>2+</sup>, restraining AMPK activity. Knock-down of ZO-2 significantly suppressed the anti-metastasis effects of Rotenone in OS cells. Moreover, Rotenone elevated p-AMPK and ZO-2 expression but inhibited EMT and lung metastasis in <i>vivo</i>.<b>Conclusion</b> These results provide evidence supporting an anti-metastatic effect of Rotenone. These findings support the use of Rotenone in the prevention of OS metastasis.
Authors
- Kun Jiang (ORCID: https://orcid.org/0000-0001-8531-7813)
- Huaqing Ma
- Suwei Dong (ORCID: https://orcid.org/0000-0001-5081-6049)
- Zhen Li (ORCID: https://orcid.org/0000-0001-5926-7832)
- Xiang Ma (ORCID: https://orcid.org/0000-0001-7246-3360)
- Zuozhang Yang (ORCID: https://orcid.org/0000-0001-9885-5151)
- Jianqiang Wang (ORCID: https://orcid.org/0000-0002-6232-9106)
- Weiquan Wang (ORCID: https://orcid.org/0000-0001-5137-5580)
- Yunqing Wang (ORCID: https://orcid.org/0000-0002-7911-2608)
- Ziqiang Zhu (ORCID: https://orcid.org/0000-0002-5328-636X)
- Chen Bao
Institutions
- Xuzhou Medical College (CN)
- Kunming Medical University (CN)
Publication Details
- Journal
- Redox Report
- Published
- 2025-04-17
- DOI
- https://doi.org/10.1080/13510002.2025.2493556
- Citations
- 2
- Primary Topic
- Bioactive natural compounds
- Type
- article
- Field-Weighted Citation Impact
- 1.61
Funders
- National Natural Science Foundation of China
- Natural Science Foundation of Jiangsu Province